The number and variety of protein therapeutics have increased dramatically in recent years, and they now play a significant role in various fields of medicine. To satisfy therapeutic demand, these molecules must be produced at large scale in mammalian or bacterial cell hosts; but with the greater variety in protein therapeutic molecules, comes greater challenges in purifying complicated molecules to a level of quality that is required, with a process that is scalable. In this webinar, the featured speaker presents process development approaches used to overcome challenges in the recovery of a bioactive multi-unit fusion protein. Overexpression of proteins in a bacterial host often leads to the formation of inclusion bodies, which presents a major challenge for large scale recovery of bioactive material; the process of purifying proteins from inclusion bodies can be laborious and the yields are often low. A greater challenge is the need to recover bioactive proteins with a high content of cysteines. The formation of random intra- and intermolecular disulfide bonds during protein biogenesis can cause many challenges to the purification of pure, active protein, including protein misfolding, oligomerization and aggregation. Join this webinar to learn about challenges faced during the downstream process development of a complicated fusion protein and how to address them.

⌛ Event Dates & Venue

06 Dec 2022, 12:00 pm - 1:00 pm
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Ticket Pricing:
FREE

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